SAGA Transcriptional Coactivator Complex

Nara. C. Marella

Project Collaborators

• Maciej Zaczek PhD
• Paul Batty PhD
• Davide Seruggia PhD
• Molecular Discovery Platform of CeMM

Histone Modifying Complexes

• SAGA
• BAF
• MEDIATOR
• NuRD
• ATAC, PCAF, TFTC, TFIID etc.

MYC-driven Cancer

• MYC proteins are master regulators of cellular programmes
• Aberrations or upregulation of MYC-related pathways occur in the vast majority of cancers
• MYC signalling enable tumour cells to dysregulate microenvironment & evade host immune response

SAGA Complex in MYC-driven Cancers

• MYC members (GCN5) difficult to target with small-molecule inhibitors
• But Acetyltransferase- and Bromo- domains in GCN5 can be targetted
• GCN5 is part of two distinct Histone-modifying complexes, SAGA and ATAC.
• further reading Targeting histone modifying enzymes for cancer therapy [Mustachio et. al.]

Spectronaut

• Spectral Library from RAW files of combined (Control and Treatment) conditions
• Higher mass tolerance
• no default imputation
• default normalization
• PG Quanity export

Thank you!

Any questions?